Nephelometry v differential agglutination titre in the measurement of rheumatoid factors.
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Nephelometry v differential agglutination titre in the measurement of rheumatoid factors.
IgM rheumatoid factors have been measured in 92 patients by both laser nephelometry and the traditional hospital procedure of a latex slide test followed by differential agglutination titre (DAT). Of the results 86% were in accordance by both methods, but seronegative patients with a high nephelometry score all showed, after reference to their clinical state and previous investigations, that th...
متن کاملDifferential Agglutination Titre (D.A.T.) in Juvenile Rheumatoid Arthritis.
The technique employed from 1949-1956, which has been described by one of us (Scott, 1952), was a slight modification of the method originally proposed by Rose, Ragan, Pearce, and Lipman (1948). Since 1956, a further modification has been employed, as suggested by Greenbury (1957), using Perspex trays (M.R.C. pattern) in place of tubes and racks. This method will be described in detail elsewher...
متن کاملComparison of Differential Agglutination Titre (D.A.T.) in Juvenile and Adult Rheumatoid Arthritis.
In the preceding paper (Bywaters, Carter, and Scott, 1959), it was shown that the Rose-Waaler test (positive in about 50 to 70 per cent. of adult rheumatoid arthritis cases) is frequently negative in childhood arthritis (Still's disease). This has also been remarked by others (Pike, Sulkin, and Coggeshall, 1955; Alexander and de Forest, 1954; Ziff, 1957), although by the inhibition technique 95...
متن کاملComparative Measurement of Rheumatoid Factor in Serum and Synovial Fluid of Rheumatoid Arthritis Patients by ELISA and Latex-Agglutination Test
Rheumatoid factors (RF) are autoantibodies with specificity for the Fc portion of human IgG. Production of RF is a characteristic feature of rheumatoid arthritis (RA) and is detectable in high titer in about 90% of these patients. In this study, we measured total IgM, IgA, IgMRF and IgARF in serum and synovial fluid of 45 RA patients by ELISA and the results were compared with those obtained b...
متن کاملProspective comparison of laser nephelometry with standard agglutination techniques for detection of rheumatoid factor.
IgM rheumatoid factor was assayed by three routine methods: latex fixation; haemagglutination; and end point laser nephelometry in 69 patients with definite or classical rheumatoid arthritis and 58 patients with other non-rheumatoid arthropathies, selected prospectively according to the American Rheumatism Association clinical criteria. The operators of the assays were unaware of the clinical d...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1981
ISSN: 0021-9746
DOI: 10.1136/jcp.34.4.396